DNA
Part:BBa_K4438101:Design
Designed by: Neha Adarsh Group: iGEM22_IISER-Tirupati_India (2022-10-11)
FASTmiR-222-D2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 50
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 50
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 50
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 50
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The split position of the complementary sequence in the sensor is ACCCAGTAGCC....AGAGTGAGCT
Source
References: Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130