DNA

Part:BBa_K4438101:Design

Designed by: Neha Adarsh   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)


FASTmiR-222-D2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The split position of the complementary sequence in the sensor is ACCCAGTAGCC....AGAGTGAGCT



Source

References: Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130


References